What is a single-stranded DNA ladder, and how do I use Simplex’s ladders?

Our single-stranded DNA ladders are used as size standards, functioning similarly to typical double-stranded DNA ladders. They are ideal for any experiment or procedure involving single-stranded DNA or RNA. All of our single-stranded DNA ladders are shipped with a product insert that includes detailed instructions on how to use our single-stranded DNA ladders. In general, Simplex’s single-stranded DNA ladders function best in polyacrylamide gel when stained with SYBR Gold Nucleic Acid Gel Stain. Each tube of our single-stranded DNA ladder products includes approximately 12-16 uses.

What is the advantage of Simplex’s single-stranded DNA ladders over conventional double-stranded DNA ladders?

Double-stranded DNA ladders are not particularly accurate size controls for single-stranded DNA or RNA. Single-stranded and double-stranded oligonucleotides have different electrophoretic mobility characteristics, so an appropriate ladder of the same type enables more accurate sizing.

Does Simplex create custom products?

Yes. If you would like us to make a product or customize an existing product, please send us an email. If the product requested is within our manufacturing capacities, we will promptly confirm and provide an estimate on the shipping date.

Future products are in the works, including but not limited to extending our DNA ladder product line. For specific questions or inquiries, please reach out through our email or website. We look forward to working with you!